Nuclear localization of soluble CTP:phosphocholine cytidylyltransferase.

The intrinsically disordered nuclear localization signal and phosphorylation segments distinguish the membrane affinity of two cytidylyltransferase isoforms.

Membrane phosphatidylcholine homeostasis is maintained in part by a sensing device in the key regulatory enzyme, CTP:phosphocholine cytidylyltransferase (CCT). CCT responds to decreases in membrane phosphatidylcholine content by reversible membrane binding and activation. Two prominent isoforms, CCTα and -β2, have nearly identical catalytic domains and very similar membrane binding amphipathic ...

Nuclear localization of SALL4

in 2006, yamanaka et al. reported the generation of induced pluripotent stem cells (iPsc) from somatic tissue with the introduction of just 4 transcription factors: Oct4, sox2, Klf4, and c-Myc.1 The finding, with its promise of autologous therapy, was the beginning of a vast and unprecedented effort to better understand the epigenetic basis of pluripotency and of “stemness.”2 sALL4 is a zinc fi...

Finding nuclear localization signals.

A variety of nuclear localization signals (NLSs) are experimentally known although only one motif was available for database searches through PROSITE. We initially collected a set of 91 experimentally verified NLSs from the literature. Through iterated 'in silico mutagenesis' we then extended the set to 214 potential NLSs. This final set matched in 43% of all known nuclear proteins and in no kn...

Nuclear localization of mouse Mx

Predicting nuclear localization.

Nuclear localization of proteins is a crucial element in the dynamic life of the cell. It is complicated by the massive diversity of targeting signals and the existence of proteins that shuttle between the nucleus and cytoplasm. Nevertheless, a majority of subcellular localization tools that predict nuclear proteins have been developed without involving dual localized proteins in the data sets....